A critically endangered species of harlequin toad (I think Atelopus varius) has been successfully bred for the first time outside of Panama at Manchester Museum. Because of the very specific needs of this toad, the breeding enclosure has had to closely recreate the Panamanian streams that they naturally occur in.
More information can be found in the link below:
How scientists got a very rare toad to breed
More information can be found in the link below:
How scientists got a very rare toad to breed