A 25-year-old 3m long python named Melanie was among reptiles and amphibians stolen from a fauna park in northern Victoria overnight.
Thieves escaped with about half Kyabram Fauna Park's collection of 25 snakes, lizards and frogs, worth about $6000.
Park manager Ron Pope said the theft of Melanie hit particularly hard because the python had been used for educational purposes and thousands of visitors would have handled her at some stage.
"It's like losing a member of your family,'' Mr Pope said.
"Everybody is feeling Melanie's loss.''
Other animals stolen include a pair of breeding water pythons, a Murray Darling carpet python, an inland carpet python, lizards and green tree frogs.
"These people knew what they were doing - they left the venomous snakes behind,'' Mr Pope said.
He believes the snakes could be sold to private collectors.
The theft comes as the park, a not-for-profit business run by the Kyabram community, is turning the corner after enduring 13 years of drought.
"We had rebuilt the collection and now to lose these reptiles is very disheartening,'' Mr Pope said.
He said Crime Squad detectives had found footprints and fingerprints.
"I think these people are from out of town - they have cased the park and planned their raid,'' Mr Pope said.
He said the park did not have camera surveillance but he will now be reviewing security measures.