Permai’s reproductive history:
Following the first recorded natural mating in 2005, a problem developed where Permai would become scared during the mounting process and Putra Mas would become aggressive in response.
They resorted to AI and by 2009, there had been three unsuccessful AI attempts:
On the first attempt, Permai released two follicles at ovulation and was thought to have aborted the pregnancy due to the challenges of carrying twins.
On the second attempt, the semen used was of poor quality.
On the third attempt, they missed her cycle by two weeks.
Following the AI attempts, they returned to natural mating, restraining Putra Mas with a leg chain and using positive reinforcement when he mounted Permai to acclimatise them to the process. Unfortunately no successful mating ever occurred.