Thanks to the excellent work of
@TinoPup , I've been able to expand the list of Florida zoos to a whopping 119 establishments. There are many more captive wildlife facilities in the state, but I've been a little picky in assembling a list of 'accessible' locations. I haven't included anywhere that is only open for one or two days per month, or is almost entirely farm animals, or has extremely limited and expensive private tours, or is an outreach center that visits schools and businesses, etc. The 119 zoos below are all ones that are open to the public on a daily basis or are easily accessible via pre-booked tours and therefore it wouldn't be difficult to visit any of these zoos on a long Florida road trip.
119 Florida Zoos:
Alligator Attraction & Wildlife Learning Center (Madeira Beach)
Aquatica Orlando (Orlando)
Arnold’s Wildlife Rehab Center (Okeechobee)
Audubon Center for Birds of Prey (Maitland)
Back to Nature Wildlife Refuge (Orlando)
Big Cat Habitat & Gulf Coast Sanctuary (Sarasota)
Big Cat Rescue (Tampa)
Bishop Museum of Science and Nature (Bradenton)
Boyett’s Citrus Attraction (Brooksville)
Brevard Zoo (Melbourne)++
Busch Gardens Tampa Bay (Tampa)++
Busch Wildlife Sanctuary (Jupiter)
Butterfly World (Coconut Creek)
Calusa Nature Center & Planetarium (Fort Myers)
Carson Springs Wildlife Conservation Foundation (Gainesville)
Catty Shack Ranch (Jacksonville)
Central Florida Animal Reserve (St. Cloud)
Central Florida Zoo (Sanford)++
Clearwater Marine Aquarium (Clearwater)
Conservancy of Southwest Florida (Naples)
Croc Encounter (Tampa)
Dade City’s Wild Things (Dade City)
Daggerwing Nature Center (Boca Raton)
Discovery Cove (Orlando)++
Disney’s Animal Kingdom (Lake Buena Vista)++
Disney’s Animal Kingdom Lodge (Lake Buena Vista)
Dolphin Connection (Duck Key)
Dolphin Research Center (Grassy Key)
Dolphins Plus: Bayside (Key Largo)
Dolphins Plus: Marine Mammal Responder (Key Largo)
Emerald Coast Wildlife Refuge Zoological Park (Crestview)
Everglades Alligator Farm (Homestead)
Everglades Holiday Park (Fort Lauderdale)
Everglades Outpost Wildlife Rescue (Homestead)
Everglades Safari Park (Miami)
Everglades Wonder Gardens (Bonita Springs)
Flamingo Gardens (Davie)
Florida Aquarium (Tampa)++
Florida International Teaching Zoo (Bushnell)
Florida Keys Aquarium Encounters (Marathon)
Florida Museum of Natural History: Butterfly Rainforest (Gainesville)
Florida Oceanographic Coastal Center (Stuart)
Gatorama (Palmdale)
Gator Golf Adventure Park (Orlando)
Gatorland (Orlando)
GatorWorld (Wildwood)
Genesis Zoological Center (Auburndale)
Giraffe Ranch (Dade City)
Golf N Gator (Cape Canaveral)
Green Cay Nature Center (Boynton Beach)
Gulfarium Marine Adventure Park (Fort Walton Beach)
Gulf Breeze Zoo (Gulf Breeze)
Gulf Specimen Marine Lab (Panacea)
Gulf World Marine Park (Panama City Beach)
Gumbo Limbo Nature Center (Boca Raton)
Hardee County Wildlife Refuge (Zolfo Springs)
Homosassa Springs Wildlife State Park (Homosassa)
IMAG: History and Science Center (Fort Myers)
Island Dolphin Care (Key Largo)
Jacksonville Zoo (Jacksonville)++
John Pennekamp Coral Reef State Park Visitor Center & Aquarium (Key Largo)
Jungle Adventures (Christmas)
Jungle Island (Miami)
Key West Aquarium (Key West)
Key West Butterfly & Nature Conservatory (Key West)
Lion Country Safari (Loxahatchee)++
Lions, Tigers & Bears, Inc. (Arcadia)
Loggerhead Marinelife Center (Juno Beach)
Lubee Bat Conservancy (Gainesville)
Marineland Dolphin Adventure (St. Augustine)
Marine Science Center (Ponce Inlet)
McCarthy’s Wildlife Sanctuary (West Palm Beach)
Miami Seaquarium (Miami)
Monkey Jungle (Miami)
Mote Marine Laboratory & Aquarium (Sarasota)++
Naples Zoo (Naples)++
Obloy Family Ranch (Merritt Island)
Octagon Wildlife Sanctuary (Punta Gorda)
Ostego Bay Foundation (Fort Myers Beach)
Palm Beach Zoo (West Palm Beach)++
Panther Ridge Conservation Center (Loxahatchee)
Peace River Wildlife Center (Punta Gorda)
Phillip and Patricia Frost Museum of Science (Miami)
Reptile Discovery Center (De Land)
Reptile World Serpentarium (Saint Cloud)
River Center (Jupiter)
Safari Edventure (Miami)
Safari Wilderness (Lakeland)
Santa Fe College Teaching Zoo (Gainesville)++
Sarasota Jungle Gardens (Sarasota)
Save Our Seabirds: Wild Bird Learning Center (Sarasota)
Seacrest Wolf Preserve (Chipley)
Sea Life Orlando (Orlando)
SeaQuest Aquarium (Fort Lauderdale)
Seaside Seabird Sanctuary (Indian Shores)
Sea Turtle Hospital (Marathon)
SeaWorld Orlando (Orlando)++
Shell Factory & Nature Park (North Fort Myers)
Single Vision (Melrose)
Smooth Waters Wildlife Park (De Leon Springs)
South Florida Science Center and Aquarium (West Palm Beach)
St. Augustine Alligator Farm Zoological Park (St. Augustine)++
St. Augustine Aquarium (St. Augustine)
St. Lucie County Aquarium (Fort Pierce)
Suncoast Primate Sanctuary (Palm Harbor)
Tallahassee Museum (Tallahassee)
Tarpon Springs Aquarium (Tarpon Springs)
Theater of the Sea (Islamorada)
The Seas with Nemo & Friends (Epcot at Lake Buena Vista)++
Tree Hill Nature Center (Jacksonville)
Two Tails Ranch (Williston)
White Oak Conservation Center (Yulee)
Wild Florida (Kenansville)
Wildlife Survival Sanctuary (Spring Hill)
Wooten’s Animal Sanctuary & Alligator Park (Ochopee)
Zoological Wildlife Foundation (Miami)
Zoo Miami (Miami)++
ZooTampa at Lowry Park (Tampa)++
ZooWorld (Panama City Beach)
++ 17 zoos in Florida are AZA-accredited
Aquariums are in bold