Both São Paulo and Rio have a aquarium and a zoo ( plus instituto Butantan on SP, which is considered a zoo).
Hello.
Rio de Janeiro has two zoos (BioParque do Rio and Arca do Noah (this one is more like a mix between a zoo and a farm park, to be honest, but is is a member of our own version of the AZA), a farm park (Fazendinha Rio) and an aquarium (AquaRio).
Aparecida, home to the world's second largest church (behind Saint Peter's Basilica) has a small aquarium and a park with a walkthrough aviary.
São José dos Campos has (or at least used to have) a serpentarium at their main university. However, I am sure the city still has its own butterfly park.
São Paulo is home to a zoo (Zoo SP), an aquarium, an petting zoo (Na Floresta SP), a farm park (Fazendinha Estação Natureza), a herptile museum (Instituto Butantan), an insect zoo (Planeta Inseto), a butterfly park and a public park with an aquarium on its grounds (Parque da Água Branca).
There is a zoo with a theme park in Cotia (Animália Park). There is also a farm park called PetZoo.
Piracicaba has a zoo and an aquarium.
The zoo in Belo Horizonte has an aquarium on its grounds. I am quite sure there is another aquarium somewhere in the city.
Curitiba has a zoo and a public park that still has a few wild animals (Passeio Público). The latter used to be the city zoo before the 1980s, when most of the bigger creatures were moved to the current location.
Foz do Iguaçu is home to three of South America's best zoos: Refúgio Biológico Bela Vista, Parque das Aves and EcoParque Foz/Dreams EcoPark.
A zoo (Complexo Ambiental Cyro Gevaerd) and an aquarium (Oceanic Aquarium) call Balneário Camboriú home.
Aracaju has a zoo, a falcon park and an oceanarium.
Three small zoos call Belém home: Museu Paraense Emílio Goeldi (albeit this one is also a museum), Bosque Rodrigues Alves and Mangal das Garças.
Manaus has two zoos (CIGS zoo) and Bosque da Ciência.
Lisbon (Portugal) has a zoo and two aquariums (Oceanário de Lisboa and Aquário Vasco da Gama)
Porto/Vila Nova de Gaia/Avintes have three zoos in total: Zoo Santo Inácio, Parque Biológico de Gaia and SEA LIFE Porto.