I didn't realize American Flagfish was a species of pupfish until now. They're fairly common in the pet trade and I've seen them aquariums a few times.I've seen American flagfish (Jordanella floridae) at the Memphis zoo aquarium. It is a Floridian species of pupfish, as can be seen from the scientific name, and a very attractive one at that.
They still do.I believe NC Zoo has desert pupfish in the dome, or did.
Great information, according to their website they have pupfish ponds on both the Africa and Arizona trails:There's a pool of water at Phoenix Zoo that possibly still holds Desert Pupfish and it's on the Arizona Trail loop. I took this photo in 2011:
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What about the famous Devil's Hole pupfish? Did any place managed to breed them sustainably?