I tracked down all of the major American zoos that have at least 100 species of reptiles and amphibians and I've typed out a list below. Surprisingly, there are even a couple of great aquariums that each have 100 species in their collection. Other than a few exceptions where data was unavailable, this is a fairly comprehensive list and naturally includes behind-the-scenes animals. Excluded are various privately-run serpentariums and the occasional prominent facility that seems to act in secret in terms of a species list, annual attendance, etc. (I'm looking at you, Dallas World Aquarium!) but overall the numbers below should be reasonably accurate. I obtained this data from the International Zoo Yearbook and other than a few minor tweaks via some friends in the industry, here are the latest counts and Omaha leads the pack of 35 establishments even though that Nebraska facility technically doesn't even have a Reptile House. However, it does have the Lied Jungle, Desert Dome, Kingdoms of the Night, Expedition Madagascar and the Wild Kingdom Pavilion and there are terrariums scattered throughout all of those 5 buildings.
Omaha's Henry Doorly Zoo - 240 species
Bronx Zoo - 220
Nashville Zoo - 210
Dallas Zoo - 200
Saint Louis Zoo - 200
San Diego Zoo - 200
Houston Zoo - 190
Fort Worth Zoo - 170
San Antonio Zoo - 170
Oklahoma City Zoo - 165
Rio Grande Zoo - 160
Zoo Atlanta - 160
Detroit Zoo - 150
Toledo Zoo - 150
Audubon Zoo - 140
Brookfield Zoo - 140
Denver Zoo - 140
Phoenix Zoo - 135
Cincinnati Zoo - 130
Louisville Zoo - 120
Zoo Miami - 120
Tulsa Zoo - 115
Arizona-Sonora Desert Museum - 110
Gladys Porter Zoo - 110
Memphis Zoo - 110
Philadelphia Zoo - 110
Riverbanks Zoo -110
Sedgwick County Zoo - 110
Cameron Park Zoo - 100
Jacksonville Zoo - 100
Los Angeles Zoo - 100
National Aquarium at Baltimore - 100
Tennessee Aquarium - 100
Wildlife World Zoo & Aquarium - 100
Zoo Knoxville - 100
Are there any missing zoos? Any numbers that need to be updated?
Omaha's Henry Doorly Zoo - 240 species
Bronx Zoo - 220
Nashville Zoo - 210
Dallas Zoo - 200
Saint Louis Zoo - 200
San Diego Zoo - 200
Houston Zoo - 190
Fort Worth Zoo - 170
San Antonio Zoo - 170
Oklahoma City Zoo - 165
Rio Grande Zoo - 160
Zoo Atlanta - 160
Detroit Zoo - 150
Toledo Zoo - 150
Audubon Zoo - 140
Brookfield Zoo - 140
Denver Zoo - 140
Phoenix Zoo - 135
Cincinnati Zoo - 130
Louisville Zoo - 120
Zoo Miami - 120
Tulsa Zoo - 115
Arizona-Sonora Desert Museum - 110
Gladys Porter Zoo - 110
Memphis Zoo - 110
Philadelphia Zoo - 110
Riverbanks Zoo -110
Sedgwick County Zoo - 110
Cameron Park Zoo - 100
Jacksonville Zoo - 100
Los Angeles Zoo - 100
National Aquarium at Baltimore - 100
Tennessee Aquarium - 100
Wildlife World Zoo & Aquarium - 100
Zoo Knoxville - 100
Are there any missing zoos? Any numbers that need to be updated?