Edmonton Valley Zoo also holds the only Mongoose Lemurs in Canada
Calgary is the only holder of Whooping Crane, Sage Grouse, and should be the only holder of African Pygmy Goose and hottentot Teal in Canada.
Assiniboine Park is the only holder of Eurasian Crane and saddle Billed stork.
Toronto also holds the only Pygmy Hippo, Babirusa, Aldabra Tortoise, Wombat, Orangutan, Great Pied Hornbill, Wrinkled Hornbill, Tomistoma, and more.
Bird Kingdom is the only holder of several species, including Great Argus, Collared Finchbill, Blue and yellow tanager, blue breasted kingfisher, red crested turaco, and more.
African lion safari holds the only Blue Throated Macaws, Monk Vultures, Yellow Headed Vultures, King Vultures, and I believe Chaco Owls and Ural Owls in Canada.
Marineland holds the only beluga whales and grey seal in Canada.