I always thought a spider walk-through would be neat. Nephila tend to stay on their webs so are perfect for that; tarantulas (e.g. male goliath bird-eating spiders) free-roaming on the floor area; rainbow jumping spiders in the vegetation.....
Not a very attractive proposal to arachnophobes of course, but walk-through enclosures and nocturnal halls for fruit bats would also be scary to a lot of people who dislike bats.
The Los Angeles County Natural History Museum has a walk-through spider exhibit every year. Here is a video tour of it conducted by the curator who puts it together. He discusses how even arachnophobes like it.
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